Several research groups have developed high-throughput imaging systems for Caenorhabditis elegans phenotypic assays and have demonstrated these systems to be highly successful in their applications, ,,. As an alternative, automated high-throughput imaging systems have emerged. However, traditional methods relying on manual analysis are a bottleneck in large-scale quantitative experiments. Phenotypic assays are crucial in genetics. It can be run on multiple platforms and easily customized to cope with new methods and requirements. The QuantWorm software is written in Java and its source code is freely available, so it does not require use of commercial software or libraries. It is an all-in-one package for quantifying multiple phenotypes. Software source code, executable programs, and sample images are available at Our software package has several advantages over current imaging systems for C. Overall, the QuantWorm software provided accurate measurements at a high speed. We then demonstrated the application of the QuantWorm software in a drug assay and a genetic assay. To evaluate the performance of our software, we compared the results of our software with manual measurements. ![]() The WormLifespan software counts the number of moving worms by using two time-lapse images the WormLocomotion software computes the velocity of moving worms the WormLength software measures worm body size and the WormEgg software counts the number of eggs. The data acquisition module collects images and videos. Currently, the software package contains one data acquisition module and four image analysis programs: WormLifespan, WormLocomotion, WormLength, and WormEgg. Our imaging system is composed of a microscope equipped with a digital camera and a motorized stage connected to a computer running the QuantWorm software package. Here we developed an automated, high-throughput computer imaging system for quantifying multiple Caenorhabditis elegans phenotypes. Furthermore, there is an increasing need for comprehensive analyses of multiple phenotypes to provide multidimensional information. Seeing 7/10, trans 5/5.Phenotypic assays are crucial in genetics however, traditional methods that rely on human observation are unsuitable for quantitative, large-scale experiments. Size 120%.Īltitude of Moon 44°, Altitude of Sun -4°. Processing in Autostakkert 2 and Maxim DL. Mount WS-180GT, Unibrain Fire-i 702 CCD b/w camera Klevtsov-Cassegrain Santel D=360mm F=5760mm, barlow 1.6x, But seeing beyond the pole with such a low Sun is a rare pleasure for which I thank Yuri and the Astronominsk team. The steep angle of observation means that little geologic interpretation can be made other than identification of younger impact craters by their crisp and bright rims. Left of center in the foreground is Mouchez, with only a few pin-pricks of illuminated hills catching the light. ![]() Two similar-sized craters, Sylvester and Lovelace, are to the left of Hermite - LRO's QuickMap identifies each of these features (and provides depths). ![]() K is the deepest hole in this scene, being about 3.9 km below the mean lunar elevation. Just beyond the North Pole is the broad-floored crater Hermite, with Rozhdestvenskiy's far, shadowed rim, cut by a bright-rimmed crater (Roz. Image by Yuri Goryachko, Mikhail Abgarian, Konstantin Morozov, Minsk, Belarus
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